Plasmid

Part:BBa_K4258046:Design

Designed by: Jake Schumacher   Group: iGEM22_NDSU   (2022-10-06)


AraC+CDS#16+B0015+DVK


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1053
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1333
    Illegal BsaI.rc site found at 1312
    Illegal BsaI.rc site found at 2420
    Illegal SapI site found at 1035


Design Notes

All parts are MoClo Golden Gate compatible with Bsa1 sites.



Source

BBa_K4258006 BBa_K4258016 MoClo Cidar Kit

References